The bioprinting g-code file is a computer program with commands necessary to print bionic pancreas. It contains information about the geometry of the printed model saved as a list of printhead movements. In our case, it is a list of about 30,000 instructions divided into movements of printheads (bioink A, bioink B and crosslinking head). The programs were optimized with many laboratory tests using dedicated bioinks. The following parameters were optimized: path shape for all heads, fill shape and density, sequence of consecutive movements, crosslinking time and speed, removal of collision sterile movements. Below, the visualizations of the printhead movement prepared from the g-code file and a fragment text content of g-code file are attached.